Anais Nin\u27s Under a glass bell : from interior fatality to psychological motion

Most of the critic ism on Anais Nin has been an overview of her entire work. Under A Glass Bell is included but, to my knowledge, no one has explored each individual story deeply enough since Nin herself recommends Under A Glass Bell as the book to begin with when reading or studying her works. Ibelieve that her recommendation is correct because Under A Glass Bell contains the nucleus of themes which she conc entrates on in all of her works, including the Diaries

Secondary memory CD8+ T cells are more protective but slower to acquire a central–memory phenotype

The formation of memory CD8 T cells is an important goal of vaccination. However, although widespread use of booster immunizations in humans generates secondary and tertiary CD8 T cell memory, experimental data are limited to primary CD8 T cell memory. Here, we show that, compared with primary memory CD8 T cells, secondary memory CD8 T cells exhibit substantially delayed conversion to a central–memory phenotype, as determined by CD62L expression and interleukin (IL)-2 production. This delayed conversion to a central–memory phenotype correlates with reduced basal proliferation and responsiveness to IL-15, although in vitro coculture with a high concentration of IL-15 is capable of inducing proliferation and CD62L upregulation. Functionally, secondary memory CD8 T cells are more protective in vivo on a per cell basis, and this may be explained by sustained lytic ability. Additionally, secondary memory CD8 T cells are more permissive than primary memory CD8 T cells for new T cell priming in lymph nodes, possibly suggesting a mechanism of replacement for memory T cells. Thus, primary and secondary memory CD8 T cells are functionally distinct, and the number of encounters with antigen influences memory CD8 T cell function

Viral Infection Results in Massive CD8+ T Cell Expansion and Mortality in Vaccinated Perforin-Deficient Mice

AbstractPerforin-mediated cytotoxicity is essential for clearance of primary LCMV infection. BALB/c-perforin-deficient (PKO) mice survived LCMV infection by deleting NP118-specific CD8+ T cells whereas vaccination of PKO mice with Listeria expressing NP118 generated a stable memory CD8+ T cell population. However, >85% of vaccinated BALB/c-PKO mice died after LCMV infection. Mortality was associated with enormous expansion of NP118-specific CD8+ T cells in both lymphoid and nonlymphoid tissues and aberrant CD8+ T cell cytokine production. Depletion of CD8+ T cells or treatment with anti-IFNγ antibody rescued vaccinated mice from mortality. Thus, perforin was essential for resistance to secondary LCMV infection, and, in the absence of perforin, vaccination resulted in lethal disease mediated by dysregulated CD8+ T cell expansion and cytokine production

Differential Role of “Signal 3” Inflammatory Cytokines in Regulating CD8 T Cell Expansion and Differentiation in vivo

Following an infection, naïve CD8 T cells are stimulated by dendritic cells (DC) displaying pathogen-derived peptides on MHC class I molecules (signal 1) and costimulatory molecules (signal 2). Additionally, pathogen-induced inflammatory cytokines also act directly on the responding CD8 T cells to regulate their expansion and differentiation. In particular, both type I interferons (IFNs) and IL-12 have been described as critical survival signals (signal 3) for optimal CD8 T cell accumulation during the expansion phase. Furthermore, expansion in numbers of antigen-specific CD8 T cells is coupled with their acquisition of effector functions to combat the infection. However, it still remains unclear whether these same cytokines also regulate the effector/memory differentiation program of the CD8 T cell response in vivo. Here, we demonstrate that defective signaling by either type I IFNs or IL-12 to the responding CD8 T cells impairs maximal expansion in response to DC immunization + CpG ODN, but neither of these cytokines is essential to regulate the effector/memory differentiation program. In addition, lack of direct IL-12 signaling to CD8 T cells accelerates the development of central memory phenotype in both primary and secondary antigen-specific memory CD8 T cells

Legendary landscapes: a cultural geography of the Paul Bunyan and Blue Ox phenomena of the North woods

Doctor of PhilosophyDepartment of GeographyKaren J. De BresLandscapes express much of who we are. Our history, thoughts, and values are all interwoven into cultural landscape features. By researching the landscape similarities and dissimilarities on the regional level, geographers are able to learn more about a people's identity. Scattered across the vast expanses of the Northwoods, residents and visitors alike are greeted by representations of a lumberjack and his blue ox. In addition to large statues of Paul Bunyan and the Blue Ox, festivals, sporting events, businesses, and public lands are all named in honor of the two folk giants. These features are so prevalent and well known that references to the region by those who live outside the Northwoods often begin by acknowledging the folk heroes. This study explains the relationship between Paul Bunyan and the Northwoods region. Focusing on the area of northern Minnesota between the towns of Bemidji and Brainerd, qualitative research methods were conducted over a four-year period (2004-2007) to better understand the phenomena. Since the 1930s, residents of the Northwoods have used Paul Bunyan and Babe the Blue Ox landscape features to celebrate symbolically the region’s golden age of logging. These representations have evolved over the years to include both public and private landscape features. Given the level of permanence of many of the items as well as the authenticity, popularity, and attachment local residents express towards Paul and Babe, cultural landscape features of the lumberjack and his blue ox will continue to be seen as an integral component of the Northwoods regional identity